Further evidence that CP-AMPARs are critically involved in synaptic tag and capture at hippocampal CA1 synapses

Molecular Brain

Heather Kang¹  John Georgiou²  Bong-Kiun Kaang³  Min Zhuo⁴  Pojeong Park⁵  Graham L. Collingridge⁶ 

[1] Department of Physiology, Faculty of Medicine, University of Toronto, 1 King’s College Circle, M5S 1A8, Toronto, ON, Canada;Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, ON, Canada;Glutamate Receptor Group, School of Physiology, Pharmacology and Neuroscience, University of Bristol, Dorothy Hodgkin Building, Whitson Street, BS1 3NY, Bristol, UK;Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, ON, Canada;School of Biological Sciences, College of Natural Sciences, Seoul National University, 08826, Seoul, Korea;School of Biological Sciences, College of Natural Sciences, Seoul National University, 08826, Seoul, Korea;Department of Physiology, Faculty of Medicine, University of Toronto, 1 King’s College Circle, M5S 1A8, Toronto, ON, Canada;School of Biological Sciences, College of Natural Sciences, Seoul National University, 08826, Seoul, Korea;Department of Physiology, Faculty of Medicine, University of Toronto, 1 King’s College Circle, M5S 1A8, Toronto, ON, Canada;Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, ON, Canada;Glutamate Receptor Group, School of Physiology, Pharmacology and Neuroscience, University of Bristol, Dorothy Hodgkin Building, Whitson Street, BS1 3NY, Bristol, UK;School of Biological Sciences, College of Natural Sciences, Seoul National University, 08826, Seoul, Korea;Department of Physiology, Faculty of Medicine, University of Toronto, 1 King’s College Circle, M5S 1A8, Toronto, ON, Canada;Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, ON, Canada;Glutamate Receptor Group, School of Physiology, Pharmacology and Neuroscience, University of Bristol, Dorothy Hodgkin Building, Whitson Street, BS1 3NY, Bristol, UK;TANZ Centre for Research in Neurodegenerative Diseases, University of Toronto, M5S 1A8, Toronto, ON, Canada;

关键词: Synaptic tag and capture;    Synapse specificity;    Synaptic efficacy;    Synaptic potentiation;    Heterosynaptic plasticity;    Metaplasticity;    Learning;    Memory;   

DOI  :  10.1186/s13041-021-00737-2

来源: Springer

【 摘 要 】

The synaptic tag and capture (STC) hypothesis provides an important theoretical basis for understanding the synaptic basis of associative learning. We recently provided pharmacological evidence that calcium-permeable AMPA receptors (CP-AMPARs) are a crucial component of this form of heterosynaptic metaplasticity. Here we have investigated two predictions that arise on the basis of CP-AMPARs serving as a trigger of STC. Firstly, we compared the effects of the order in which we delivered a strong theta burst stimulation (TBS) protocol (75 pulses) and a weak TBS protocol (15 pulses) to two independent inputs. We only observed significant heterosynaptic metaplasticity when the strong TBS preceded the weak TBS. Second, we found that pausing stimulation following either the sTBS or the wTBS for ~20 min largely eliminates the heterosynaptic metaplasticity. These observations are consistent with a process that is triggered by the synaptic insertion of CP-AMPARs and provide a framework for establishing the underlying molecular mechanisms.

【 授权许可】

CC BY