Microbial Cell Factories | |
Tunable expression rate control of a growth-decoupled T7 expression system by l-arabinose only | |
Gerald Striedner1  Patrick Stargardt2  Juergen Mairhofer2  | |
[1] Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, 1190, Vienna, Austria;enGenes Biotech GmbH, Mooslackengasse 17, 1190, Vienna, Austria; | |
关键词: Escherichia coli; enGenes-X-press; Growth-decoupled; gp2; BL21-AI; Recombinant protein production; Expression rate control; Resource reallocation; Flow cytometry; pBAD; -Arabinose; | |
DOI : 10.1186/s12934-021-01512-7 | |
来源: Springer | |
【 摘 要 】
BackgroundPrecise regulation of gene expression is of utmost importance for the production of complex membrane proteins (MP), enzymes or other proteins toxic to the host cell. In this article we show that genes under control of a normally Isopropyl β-d-1-thiogalactopyranoside (IPTG)-inducible PT7-lacO promoter can be induced solely with l-arabinose in a newly constructed Escherichia coli expression host BL21-AI
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO202106280375911ZK.pdf | 4192KB | download |