Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

eLife

Scott D Michaels¹  Pascal GP Martin¹  Xuhong Yu¹  Vasiliki Zacharaki²  Anna V Sherwood²  Katarzyna Knop²  Matthew T Parker²  Geoffrey J Barton²  Gordon G Simpson³  Jim Beynon⁴  Daniel Tomé⁴ 

[1] Department of Biology, Indiana University, Bloomington, United States;School of Life Sciences, University of Dundee, Dundee, United Kingdom;School of Life Sciences, University of Dundee, Dundee, United Kingdom;The James Hutton Institute, Invergowrie, United Kingdom;School of Life Sciences, University of Warwick, Coventry, United Kingdom;

关键词: NLR;    nanopore;    direct RNA sequencing;    spen;    m6A;    alternative polyadenylation;    A. thaliana;   

DOI  :  10.7554/eLife.65537

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.

【 授权许可】

CC BY