Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons

eLife

Rohini Kuner¹  Yvrick Zagar²  Alain Chédotal²  Eljo Van Battum²  Celine Heitz-Marchaland²  Stéphane Fouquet² 

[1] Pharmacology Institute, Heidelberg University, Heidelberg, Germany;Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France;

关键词: cerebellum;    semaphorins;    cell migration;    cytokinesis;    Mouse;   

DOI  :  10.7554/eLife.60554

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Plexin-B2 deletion leads to aberrant lamination of cerebellar granule neurons (CGNs) and Purkinje cells. Although in the cerebellum Plexin-B2 is only expressed by proliferating CGN precursors in the outer external granule layer (oEGL), its function in CGN development is still elusive. Here, we used 3D imaging, in vivo electroporation and live-imaging techniques to study CGN development in novel cerebellum-specific Plxnb2 conditional knockout mice. We show that proliferating CGNs in Plxnb2 mutants not only escape the oEGL and mix with newborn postmitotic CGNs. Furthermore, motility of mitotic precursors and early postmitotic CGNs is altered. Together, this leads to the formation of ectopic patches of CGNs at the cerebellar surface and an intermingling of normally time-stamped parallel fibers in the molecular layer (ML), and aberrant arborization of Purkinje cell dendrites. There results suggest that Plexin-B2 restricts CGN motility and might have a function in cytokinesis.

【 授权许可】

CC BY