The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling

eLife

Bing Chen¹  Chao Wang¹  Meng-Qiu Dong²  Yong-Hong Yan²  Shi-Li Xia³  Yingchuan B Qi³  Meng Li³ 

[1] College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China;National Institute of Biological Sciences, Beijing, China;School of Life Science and Technology, ShanghaiTech University, Shanghai, China;College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China;

关键词: synaptic rewiring;    laval development;    MYRF;    pan-1;    membrane-bound;    transcription factors;    C. elegans;   

DOI  :  10.7554/eLife.67628

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Neural circuits develop through a plastic phase orchestrated by genetic programs and environmental signals. We have identified a leucine-rich-repeat domain transmembrane protein PAN-1 as a factor required for synaptic rewiring in C. elegans. PAN-1 localizes on cell membrane and binds with MYRF, a membrane-bound transcription factor indispensable for promoting synaptic rewiring. Full-length MYRF was known to undergo self-cleavage on ER membrane and release its transcriptional N-terminal fragment in cultured cells. We surprisingly find that MYRF trafficking to cell membrane before cleavage is pivotal for C. elegans development and the timing of N-MYRF release coincides with the onset of synaptic rewiring. On cell membrane PAN-1 and MYRF interact with each other via their extracellular regions. Loss of PAN-1 abolishes MYRF cell membrane localization, consequently blocking myrf-dependent neuronal rewiring process. Thus, through interactions with a cooperating factor on the cell membrane, MYRF may link cell surface activities to transcriptional cascades required for development.

【 授权许可】

CC BY