eLife | |
The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling | |
Bing Chen1  Chao Wang1  Meng-Qiu Dong2  Yong-Hong Yan2  Shi-Li Xia3  Yingchuan B Qi3  Meng Li3  | |
[1] College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China;National Institute of Biological Sciences, Beijing, China;School of Life Science and Technology, ShanghaiTech University, Shanghai, China;College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China; | |
关键词: synaptic rewiring; laval development; MYRF; pan-1; membrane-bound; transcription factors; C. elegans; | |
DOI : 10.7554/eLife.67628 | |
来源: eLife Sciences Publications, Ltd | |
【 摘 要 】
Neural circuits develop through a plastic phase orchestrated by genetic programs and environmental signals. We have identified a leucine-rich-repeat domain transmembrane protein PAN-1 as a factor required for synaptic rewiring in C. elegans. PAN-1 localizes on cell membrane and binds with MYRF, a membrane-bound transcription factor indispensable for promoting synaptic rewiring. Full-length MYRF was known to undergo self-cleavage on ER membrane and release its transcriptional N-terminal fragment in cultured cells. We surprisingly find that MYRF trafficking to cell membrane before cleavage is pivotal for C. elegans development and the timing of N-MYRF release coincides with the onset of synaptic rewiring. On cell membrane PAN-1 and MYRF interact with each other via their extracellular regions. Loss of PAN-1 abolishes MYRF cell membrane localization, consequently blocking myrf-dependent neuronal rewiring process. Thus, through interactions with a cooperating factor on the cell membrane, MYRF may link cell surface activities to transcriptional cascades required for development.
【 授权许可】
CC BY
【 预 览 】
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