Regional sequencing collaboration reveals persistence of the T12 Vibrio cholerae O1 lineage in West Africa

eLife

Serges Tchatchouang¹  Ariane Nzouankeu¹  Sani Ousmane²  Ibrahim Dan Dano²  Bassira Boubacar Issaka²  Andrew Azman³  Shirlee Wohl³  Justin Lessler³  Ifeoluwa Akintayo⁴  Francisco Luquero⁵  Yap Boum⁵  Blaise Akenji⁶  Sylvie Melingui⁶  Anthony Ahumibe⁷  Christopher Chukwu⁷  Eme Ekeng⁷  Michael Oladotun Popoola⁷  Dhamari Naidoo⁸ 

[1]Centre Pasteur du Cameroun, Yaoundé, Cameroon

[2]Centre de Recherche Médicale et Sanitaire, Niamey, Niger

[3]Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, United States

[4]Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria

[5]Epicentre, Paris, France

[6]National Public Health Laboratory, Yaoundé, Cameroon

[7]Nigeria Centre for Disease Control, Abuja, Nigeria

[8]World Health Organization Nigeria, Abuja, Nigeria

关键词: Vibrio cholerae;    genomics;    transmission;    West Africa;    antimicrobial resistance;    Other;   

DOI  :  10.7554/eLife.65159

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Background:Despite recent insights into cholera transmission patterns in Africa, regional and local dynamics in West Africa—where cholera outbreaks occur every few years—are still poorly understood. Coordinated genomic surveillance of Vibrio cholerae in the areas most affected may reveal transmission patterns important for cholera control.Methods:During a regional sequencing workshop in Nigeria, we sequenced 46 recent V. cholerae isolates from Cameroon, Niger, and Nigeria (37 from 2018 to 2019) to better understand the relationship between the V. cholerae bacterium circulating in these three countries.Results:From these isolates, we generated 44 whole Vibrio cholerae O1 sequences and analyzed them in the context of 1280 published V. cholerae O1 genomes. All sequences belonged to the T12 V. cholerae seventh pandemic lineage.Conclusions:Phylogenetic analysis of newly generated and previously published V. cholerae genomes suggested that the T12 lineage has been continuously transmitted within West Africa since it was first observed in the region in 2009, despite lack of reported cholera in the intervening years. The results from this regional sequencing effort provide a model for future regionally coordinated surveillance efforts.Funding:Funding for this project was provided by Bill and Melinda Gates Foundation OPP1195157.

【 授权许可】

CC BY