Proteomic and transcriptomic profiling reveal different aspects of aging in the kidney

eLife

Steven P Gygi¹  Joel M Chick²  Olivier Devuyst³  Isabela Gerdes Gyuricza⁴  Daniel A Skelly⁴  Ron Korstanje⁴  Yuka Takemon⁴  Gary A Churchill⁴ 

[1] Harvard Medical School, Boston, United States;Harvard Medical School, Boston, United States;VividionTherapeutics, San Diego, United States;Institute of Physiology, University of Zurich, Zurich, Switzerland;The Jackson Laboratory, Bar Harbor, United States;

关键词: mouse;    kidney;    aging;    mRNA;    protein;    Mouse;   

DOI  :  10.7554/eLife.62585

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Little is known about the molecular changes that take place in the kidney during the aging process. In order to better understand these changes, we measured mRNA and protein levels in genetically diverse mice at different ages. We observed distinctive change in mRNA and protein levels as a function of age. Changes in both mRNA and protein are associated with increased immune infiltration and decreases in mitochondrial function. Proteins show a greater extent of change and reveal changes in a wide array of biological processes including unique, organ-specific features of aging in kidney. Most importantly, we observed functionally important age-related changes in protein that occur in the absence of corresponding changes in mRNA. Our findings suggest that mRNA profiling alone provides an incomplete picture of molecular aging in the kidney and that examination of changes in proteins is essential to understand aging processes that are not transcriptionally regulated.

【 授权许可】

CC BY