Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris

eLife

Nilasha Sen¹  Renee C Zhang¹  Connor R Finkbeiner²  Brent A Wilkerson²  Olivia Bermingham-McDonogh²  Heather L Zebroski²  Kylie E Beach²  Alex D Chitsazan³ 

[1] Department of Biological Structure, University of Washington, Seattle, United States;Department of Biological Structure, University of Washington, Seattle, United States;Institute for Stem Cells and Regenerative Medicine, University of Washington, Seattle, United States;Department of Biological Structure, University of Washington, Seattle, United States;Institute for Stem Cells and Regenerative Medicine, University of Washington, Seattle, United States;Department of Biochemistry, University of Washington, Seattle, United States;

关键词: inner ear;    vestibular;    sensory;    single cell;    hair cells;    Mouse;   

DOI  :  10.7554/eLife.60108

来源: eLife Sciences Publications, Ltd

【 摘 要 】

This study provides transcriptomic characterization of the cells of the crista ampullaris, sensory structures at the base of the semicircular canals that are critical for vestibular function. We performed single-cell RNA-seq on ampullae microdissected from E16, E18, P3, and P7 mice. Cluster analysis identified the hair cells, support cells and glia of the crista as well as dark cells and other nonsensory epithelial cells of the ampulla, mesenchymal cells, vascular cells, macrophages, and melanocytes. Cluster-specific expression of genes predicted their spatially restricted domains of gene expression in the crista and ampulla. Analysis of cellular proportions across developmental time showed dynamics in cellular composition. The new cell types revealed by single-cell RNA-seq could be important for understanding crista function and the markers identified in this study will enable the examination of their dynamics during development and disease.

【 授权许可】

CC BY