GABAA presynaptic inhibition regulates the gain and kinetics of retinal output neurons

eLife

Jenna Nagy¹  Raunak Sinha²  Mrinalini Hoon²  Briana Ebbinghaus³ 

[1] Department of Neuroscience, University of Wisconsin, Madison, United States;McPherson Eye Research Institute, University of Wisconsin, Madison, United States;Cellular and Molecular Pathology Training Program, University of Wisconsin, Madison, United States;Department of Neuroscience, University of Wisconsin, Madison, United States;McPherson Eye Research Institute, University of Wisconsin, Madison, United States;Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, United States;McPherson Eye Research Institute, University of Wisconsin, Madison, United States;Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, United States;Neuroscience Training Program, University of Wisconsin, Madison, United States;

关键词: retina;    GABA receptor;    bipolar cell;    ganglion cell;    presynaptic inhibition;    Mouse;   

DOI  :  10.7554/eLife.60994

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Output signals of neural circuits, including the retina, are shaped by a combination of excitatory and inhibitory signals. Inhibitory signals can act presynaptically on axon terminals to control neurotransmitter release and regulate circuit function. However, it has been difficult to study the role of presynaptic inhibition in most neural circuits due to lack of cell type-specific and receptor type-specific perturbations. In this study, we used a transgenic approach to selectively eliminate GABAA inhibitory receptors from select types of second-order neurons – bipolar cells – in mouse retina and examined how this affects the light response properties of the well-characterized ON alpha ganglion cell retinal circuit. Selective loss of GABAA receptor-mediated presynaptic inhibition causes an enhanced sensitivity and slower kinetics of light-evoked responses from ON alpha ganglion cells thus highlighting the role of presynaptic inhibition in gain control and temporal filtering of sensory signals in a key neural circuit in the mammalian retina.

【 授权许可】

CC BY