Separation of autologous red blood cells from specimen of the transfused sickle cell disease patients using hypotonic saline

Global Journal of Transfusion Medicine

article

Ankita Sheladiya¹  Kinjal Mendapara-Dobariya¹  Sanmukh R Joshi¹ 

[1] Lok Samarpan Regional Blood Bank and Research Center

关键词: Autologous red blood cells;    sickle cell disease;    transfused patients;   

DOI  :  10.4103/GJTM.GJTM_1_17

学科分类：社会科学、人文和艺术（综合）

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Background: Red blood cell (RBC) antigen typing in transfused sickle cell disease (SCD) patients is a difficult task due to contamination of the transfused red cells. Aim: This pilot study was aimed to standardize an easy approach to separate the autologous RBCs from the transfused patient for RBC antigen typing in SCD. Materials and Methods: The RBCs from transfused patients with SCD were separated by hypotonic saline and tested with various antisera by standard serological methods. Results: Ten antisera showing negative reaction with donors' RBCs reacted positively with the patients' autologous RBCs separated by treatment with hypotonic saline. Seven antisera reacted with the donors' RBCs were nonreactive with the patients' autologous RBCs after elimination of the donor's RBCs by treatment with hypotonic saline. Conclusion: The hypotonic saline can be used as method to separate the autologous RBCs from the mixture with the transfused RBC in patients with SCD.

【 授权许可】

CC BY-NC-SA