Detection of Coxiella burnetii and sequencing the IS1111 gene fragment in bulk tank milk of dairy herds

The Iranian Journal of Veterinary Science and Technology

article

Borji, Shiva¹  Jamshidi, Abdollah²  Khanzadi, Saeid²  Razmyar, Jamshid³ 

[1] Faculty of Veterinary Medicine, Ferdowsi University of Mashhad;Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad;Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad

关键词: Bulk tank milk;    Coxiella burnetii;    Touchdown PCR;   

DOI  :  10.22067/veterinary.v6i2.26330

学科分类：医学（综合）

来源: Ferdowsi University of Mashhad

【 摘 要 】

Coxiella burnetii is the etiologic agent of Q fever in human. This study is aimed to determine the contamination rate of C. burnetii in bulk tank milk samples. In this study, a total number of 100 bulk milk samples collected from dairy farms in northeast of Iran and were examined for C. burnetii using touchdown PCR assay. The primers were designed to amplify a 687-bp fragment of the IS1111 gene of C. burnetii. Five samples (5%) of bovine milk were found positive for C. burnetii. Sequence analysis of PCR products revealed 100% identity with published sequences of C. burnetii in genBank. Results of this study indicate that bovine milk is an important source of C. burnetii infection in Iran.

【 授权许可】

CC BY