In Vitro Studies of Jatropha curcas L. Latex Spray Formulation for Wound Healing Applications

Turkish Journal of Pharmaceutical Sciences

article

Kittiya TINPUN¹  Titpawan NAKPHENG¹  Alwar Ramanujam PADMAVATHI¹  Teerapol SRICHANA² 

[1] Prince of Songkla University, Drug Delivery System Excellence Center;Prince of Songkla University, Drug Delivery System Excellence Center, Thailand & Prince of Songkla University Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Technology

关键词: Jatropha curcas L;    latex;    wound healing;    spray formulation;    collagen production;    antioxidant;   

DOI  :  10.4274/tjps.galenos.2019.69875

来源: Galenos Yayinevi

【 摘 要 】

Objectives: There is an increasing demand for wound healing products of natural origin. Our objective was to develop a spray formulation from Jatropha curcas (J. curcas) L. latex extracts for wound healing applications. Materials and Methods: J. curcas L. latex was subjected to solvent extraction. The phytochemical structure was elucidated by 1 H-NMR and confirmed by liquid chromatography-mass spectrometer spectrometry. A topical spray formulation prepared from J. curcas latex extracts was evaluated in terms of its antimicrobial activity and radical scavenging activity. The toxicity of the formulation on fibroblast cell lines, collagen production, and wound healing activities were tested. Results: The 1 H-NMR and mass spectrometric analyses revealed the pure compound as curcacycline A. The J. curcas latex extract formulation had radical scavenging and antibacterial activities. Moreover, the formulation was not toxic to the human fibroblast cells and it stimulated collagen production and healed cell injury in 24 h. Conclusion: The J. curcas latex extract promoted wound healing after cell injury. Our findings indicate the possibility of utilizing the J. curcas latex extract spray formulation as a potential antibacterial, antioxidant, and wound healing product from nature.

【 授权许可】

Unknown