Comparative In Vitro Assessment of the Methanol Extracts of the Leaf, Stem, and Root Barks of Cnidoscolus aconitifolius on Lung and Breast Cancer Cell Lines

Turkish Journal of Pharmaceutical Sciences

article

Emmanuel Oise OIKPEFAN¹  Buniyamin Adesina AYINDE²  Azhar MUDASSIR³  Ahsana Dar FAROOQ³ 

[1] Delta State University, Faculty of Pharmacy, Department of Pharmacognosy and Traditional Medicine;University of Benin, Faculty of Pharmacy, Department of Pharmacognosy;University of Karachi, International Centre for Chemical and Biological Sciences, Dr. Panjwani Center for Molecular Medicine and Drug Research

关键词: Cytotoxicity;    Cnidoscolus aconitifolius;    growth inhibition;    MCF-7;    NCI-H460;    cancer cells;   

DOI  :  10.4274/tjps.galenos.2018.19942

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Cnidoscolus aconitifolius Mill. I.M.Johnst. is a medicinal plant widely used in ethnomedicine for the treatment of cancer and other diseases. Materials and Methods: The effects of methanol extracts of the leaf, stem, and root barks were evaluated on breast (MCF-7) and lung (NCI-H460) cancer cells at 1-250 µg/mL using the SRB assay and the extracts were screened for phytochemicals using the standard method. Results: The stem and root extracts showed no activity at the maximum concentration, while the leaf extract at 100 µg/mL showed remarkable cell growth inhibition against breast (-14.50±0.58) and lung cancer (+53.29±4.57) in vitro. The extracts showed the presence of saponins, terpenes, cardiac glycosides, and phenolic compounds. Partitioning of the active leaf extract further enhanced its activity as the chloroform fraction exhibited GI50, LC50, and total growth inhibition (TGI) of 22.5, 68.75, and 43.75 µg/mL against breast cancer, respectively, and GI50 and TGI of 35.4 and 55.8 µg/ mL against lung cancer cells, respectively. However, the aqueous fraction showed no cytotoxicity against either cell line. Conclusion: These results justified the ethnomedicinal uses of the plant against tumor-related ailments. Isolation of the constituents responsible for the observed activity needs to be carried out to further support this claim.

【 授权许可】

Unknown