Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of Artemisia campestris from Algeria

Turkish Journal of Pharmaceutical Sciences

article

Boulanouar BAKCHICHE¹  Abdelaziz GHERIB¹  Maria Rosário BRONZE²  Mosad A. GHAREEB³ 

[1] Amar Telidji University, Faculty of Technology, Laboratory of Process Engineering;University of Lisbon, Faculty of Pharmacy;Medicinal Chemistry Department, Theodor Bilharz Research Institute

关键词: Artemisia campestris;    polyphenols;    flavonoids;    chlorogenic acid;    antioxidant;   

DOI  :  10.4274/tjps.galenos.2018.99267

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Our study aimed to investigate the chemical profile of hydroalcoholic extract of Algerian Artemisia campestris and its antioxidant activity. Materials and Methods: The hydroalcoholic extract of Algerian A. campestris was investigated for its phenolic constituents using high performance liquid chromatography (HPLC)-diode array detection (DAD)-electrospray ionization (ESI)-mass spectrometer (MS)/MS. The in vitro antioxidant activity and total phenolic content were also evaluated via oxygen radical absorbance capacity and Folin–Ciocalteu assays, respectively. Results: HPLC-DAD-ESI-MS/MS analysis revealed that the main tentatively identified compounds were caffeoylquinic acid isomers, flavonoids, and benzoic acid derivatives. Additionally, the hydroalcoholic extract exhibited a promising antioxidant activity value of 120.5±10.4 μmol Trolox equivalent antioxidant capacity/g dry weight (DW), and a strong correlation exists between this activity and the total phenolic content value of 102.09±1.65 mg/g gallic acid equivalents DW. Conclusion: The hydroalcoholic extract of A. campestris is a promising candidate for the production of naturally occurring antioxidant agents.

【 授权许可】

Unknown