Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Turkish Journal of Pharmaceutical Sciences

article

Merve BACANLI¹  Hatice Gül ANLAR¹  A. Ahmet BAŞARAN²  Nursen BAŞARAN¹ 

[1] Hacettepe University, Faculty Of Pharmacy, Department Of Pharmaceutical Toxicology;Hacettepe University, Faculty Of Pharmacy, Departments Of Pharmacognosy

关键词: MTT;    neutral red;    plant phenolics;   

DOI  :  10.4274/tjps.07078

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Phenolic compounds exhibit several health protective properties. Galangin, curcumin, pycnogenol, puerarin and ursolic acid are commonly used plant phenolics in folk medicine. The aim of our study was to evaluate the difference between neutral red uptake (NRU) and MTT assays using different plant phenolics (galangin, curcumin, pycnogenol, puerarin and ursolic acid) in healthy and cancer cells in different time periods. Materials and Methods: In this study, the cytotoxic effects of these phenolic compounds were investigated by NRU and MTT assays in healthy (V79, Chinese hamster fibroblast cell line) and cancer [human cervix epithelial adenocarcinoma cell line Henrietta Lacks (HeLa) and human mammary carcinoma cell line (BT-474)] in 18, 24 and 48 h incubation periods. Results: Our results demonstrated that galangin, curcumin, pycnogenol, puerarin and ursolic acid decreased cell viability of V79, HeLa and BT-474 cells in a dose-dependent manner in 18, 24 and 48 h incubation periods. However, the cell survival rate was much lower in 48 h incubation period. There was no difference between the results from NRU and MTT assays. Conclusion: To decide which incubation period and which cytotoxicity study to be used, the cytotoxicity mechanism of the compound must be known.

【 授权许可】

Unknown