Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity

Journal of Medicinal Plants for Economic Development

ARTICLE

Enitome E. Bafor¹  Wellington O. Omokaro¹  Osamuyi H. Uwumarongie²  Uloma B. Elvis-Offiah³  Osemelomen Omoruyi¹  Christina V. Viegelmann⁴  RuAngelie Edrada-Ebel⁴ 

[1] Department of Pharmacology & Toxicology, University of Benin;Department of Pharmacognosy, University of Benin;Department of Science and Laboratory Technology, University of Benin;Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde

关键词: Dryopteris filix-mas;    uterus;    oxytocin;    fatty acids;    porphyrins;   

DOI  :  10.4102/jomped.v1i1.25

学科分类：自然科学（综合）

来源: AOSIS

【 摘 要 】

Background:  The plant  Dryopteris filix-mas  has been used traditionally for its uterine-stimulant effects. Aim:  The current study is therefore aimed at investigating and determining the effect of the leaves of  D. filix-mas  on uterine contractility  in vitro . Setting:  Fresh leaves of  D. filix-mas  were collected from a river bank in the south-western part of Nigeria. Methods:  The leaves of  D. filix-mas  were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites. Results:  The plant extract inhibited spontaneous-induced contractions with IC 50  amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC 50  frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions ( p  < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified. Conclusion:  The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.

【 授权许可】

CC BY