期刊论文详细信息
ISCC (Indonesian Journal of Cancer Chemoprevention) | |
Pentagamavunon-0 (PGV-0) Enhance Cytotoxic Effect of Doxorubicin through Increasing of Apoptosis, Senescence and ROS Level on Triple Negative Breast Cancer 4T1 | |
ARTICLE | |
Ismanurrahman Hadi1  Riris Istighfari Jenie2  Edy Meiyanto2  | |
[1]Magister Program of Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada | |
[2]Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada | |
[3]Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada | |
关键词: 4T1; PGV-0; Co-chemotherapy; Cytotoxic; Senescence; Apoptosis; ROS; | |
DOI : 10.14499/indonesianjcanchemoprev11iss1pp7-15 | |
学科分类:药学 | |
来源: Indonesian Society for Cancer Chemoprevention | |
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【 摘 要 】
TNBC, one of the sub type of breast cancers was widely known with high tumorigenic and poor prognosis than others. The development of combination agent (co-chemotherapy) with doxorubicin for chemotherapy of TNBC were carried out to decrease doxorubicin side effect and resistance in cancer. This present study aims to explore the co-chemotherapeutic properties of PGV-0 and investigate induction of doxorubicin on apoptosis, senescence and ROS against TNBC. 4T1 Cell line were used as a TNBC in vitro model. Cytotoxic measurement was performed using MTT assay resulting in IC50 values of 52 μM. Meanwhile, the combination of doxorubicin and PGV-0 showed synergistic effect which decreased cell viability of 4T1 better than single treatment of doxorubicin. Apoptosis analysis was performed using annexin V/PI assay indicated that the combination treatment of PGV-0 and doxorubicin increased apoptosis evidence. Senescence detection was carried out using senescence-associated-β galactosidase (SA-β-gal) assay. The results showed that a single treatment of PGV-0 induced cellular senescence and increased senescence cells in combination treatment. Moreover, DCFDA staining showed that PGV-0 increased ROS level at single treatment, whereas combination treatment increased ROS intracellular compared to the positive control of doxorubicin. Based on these results, PGV-0 has potential as a co-chemotherapeutic candidate on TNBC.【 授权许可】
CC BY-NC
【 预 览 】
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