Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Yurista Gilang¹  Adam Hermawan²  Aditya Fitriasari¹  Riris Istighfari Jenie² 

[1] Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada

关键词: hesperidin;    5-fluorouracil;    WiDr cells;    cytotoxic;    apoptosis;   

DOI  :  10.14499/indonesianjcanchemoprev3iss2pp404-409

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

Therapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC 50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation. Either single treatment of hesperidin 200µM or 5-FU 1500 µM did not trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.

【 授权许可】

CC BY-NC