Antiproliferative Effect of Chloroform Extract of Cangkring Leaves (Erythrina fusca Lour.) and Its Isolates against HeLa Cells

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Endah Puspitasari¹  Endah Dwi Setyowati²  Binar Asrining Dhiani³  Sugeng Riyanto⁴ 

[1]Faculty of Pharmacy, Universitas Jember

[2]RSUP Dr. Sardjito

[3]Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto

[4]Faculty of Pharmacy, Universitas Gadjah Mada

关键词: Erythrina fusca Lour.;    isolate and effluent of chloroform extract;    antiproliferative;    cervical cancer;   

DOI  :  10.14499/indonesianjcanchemoprev3iss1pp358-363

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

Cervical cancer is a type of cancer possessing the 3 rd highest incidence among women worldwide. Today’s therapies against cancer are still ineffective, thus people are seeking for alternative method. Erythrina fusca Lour. has been used traditionally as anticancer. This experiment is conducted to study the ability of chloroform extract of E. fusca Lour. leaves and its isolates on HeLa cervical cancer cells. The cytotoxicity assay and doubling time assay were done using direct counting method, while the DNA staining assay was done using acrydine orange. The chloroform extract and isolate # 30 showed cytotoxic activity with IC 50 of 16 and 5 µg/ml, respectively, while isolate # 2 and isolate # 8 didn’t. The effluent # 30 could not affect HeLa cells growth at the given concentration, but it might promote DNA fragmentation indicating apoptosis induction. The molecular mechanisms underlying these effects still need to be further explored.

【 授权许可】

CC BY-NC