In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Tati Herlina¹  Unang Supratman¹  Anas Subarnas²  Supriyatna Sutardjo²  Suseno Amien³  Hideo Hayashi⁴ 

[1] Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University;Faculty of Pharmacy, Padjadjaran University;Faculty of Agriculture, Padjadjaran University;Laboratory of Natural Products Chemistry, Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, Osaka Prefecture University

关键词: Anti-cancer;    Erythrina variegata;    Leguminoseae;   

DOI  :  10.14499/indonesianjcanchemoprev2iss3pp286-290

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D . In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid ( 1 ) and isoflavonoid ( 2 ). Compounds ( 1 - 2 ) showed anti-cancer activity against breast cancer cell T47D used with IC 50 of  1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents.

【 授权许可】

CC BY-NC