Isolation and Structure Elucidation of Soulatro Coumarin From Stem Bark of Calophyllum soulattri Burm F and In Vivo Antiplasmodial Activity by Using Mice Infected by Plasmodium berghei

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Jamilah Abbas¹  Achmad Darmawan¹  Syafruddin Syafruddin² 

[1] Research Centre for Chemistry, Indonesian Institute of Sciences PUSPIPTEK. Serpong. 15314. Indonesia;Eijkman Institute for Molecular Biology

关键词: Antiplasmodial activity;    coumarin;    Calophyllum soulattri Burm. F;    in vivo;    Chloroquine;    Plasmodium berghei.;   

DOI  :  10.14499/indonesianjcanchemoprev2iss2pp242-254

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

The soulatro coumarin compound was isolated and elucidated from the stem bark of Calophyllum soulattri Burm F, the samples were collected from Jayapura Papua Irian Island in Indonesia. Isolation process was done by maceration at room temperature in methanol, than partitioned in a mixture of n hexane-water (1:1), followed by dichloromethane-water (1:1)  and ethyl acetate-water (1:1). A portion of ethyl acetate extract was subjected to column chromatography over silica gel packed and eluted with n -hexane a gradient of ethyl acetate to 100% followed by CHCl 3   in MeOH (20:1, 10 :1, 5:1, 1:1). Fraction  B (CHCl 3 in MeOH 20:1) was subjected to column chromatography  over silica gel 300 mesh  and eluted with EtOAc-MeOH mixtures of increasing polarity. Faction with the same R f valeus were combined and eluted with EtOAc-MeOH  (19:1) showed one spot on TLC. They were combined and evaporated to yield a solid than was recrystallized in mixture of CH 2 Cl 2 -methanol to give soulatro coumarin compound. The structure was determinated by spectroscopic analysis, in particular by 1D and 2D NMR techniques, from these spectra data conclution that compound is soulatro coumarin. Antimalarial assay was tested against Plasmodium berghei parasite as in vivo using 18 mices rodent wich was infected by  Plasmodium berghei parasite. The soulatro coumarin  showed activity against P. berghei with dosage 0.0005867 mM/1 kg body weight ; 0.005867 mM/1 kg bw; 0.05867 mM/1 kg bw; 0.5867 mM/1 kg bw 5.867 mM/1 kg bw and 58.67 mM/1 kg bw could inhibite growth rate of parasite = 57.32%; 63.37%; 43.02%; 53.49%; 47.67% respectively.

【 授权许可】

CC BY-NC