Chemopreventive Efficacy of Ginger Extract (Zingiber officinale)

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Harliansyah Harliansyah¹  W.N. Wan Zurinah²  M.Y. Yasmin Anum²  M. Noor Azian³ 

[1] Department of Biochemistry, Yarsi University School of Medicine;Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia;Centre of Lipids and Engineering and Applied Research, Universiti Teknologi Malaysia

关键词: antiproliferation;    apoptosis;    caspases;    Zingiber officinale;   

DOI  :  10.14499/indonesianjcanchemoprev1iss2pp118-123

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

The liver cells were cultured in the presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities. Ginger extract exhibited a dose dependent inhibition of viability and proliferation of WRL-68, HLE and HepG2 cells with IC 50 of 569.69 ± 7.99 µg/ml, 389.71 ± 26.56 µg/ml and 358.71 ± 17.12 µg/ml respectively. Ginger extract induced apoptosis through activation of caspase-8 and 3 in a dose dependent pattern for all cells at concentration ranging from 0-500 mg/ml.  We found that antiproliferative effect of ginger extract could be associated with induction of apoptosis as shown by increased activities of caspase 8 and 3.The results from this study suggest that ginger extract has chemopreventive properties against hepatoma cells HepG2 and HLE by inhibiting cellular proliferation and inducing apoptosis.

【 授权许可】

CC BY-NC