The Effect of Orally Administered Probiotics on the Behavioral, Cellular, and Molecular Aspects of Adjuvant-Induced Arthritis

Basic and Clinical Neuroscience

ARTICLE

Shadnoush, Mahdi¹  Nazemian, Vida²  Manaheji, Homa²  Zaringhalam, Jalal² 

[1] Department of Clinical Nutrition & Dietetics, School of Nutrition Sciences & Food Technology, Shahid Beheshti University of Medical Sciences;Neurophysiology Research Centre, Shahid Beheshti University of Medical Sciences

关键词: Probiotics;    Hyperalgesia;    Edema;    Interleukin-1β (IL-1β);    p38 Mitogen-Activated Protein Kinase (MAPK);    μ-Opioid Receptor (MOR);   

DOI  :  10.32598/bcn.9.5.325

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: Rheumatoid Arthritis (RA) is a chronic autoimmune disease, which is accompanied with pain, hyperalgesia, and edema. Overproduction of pro-inflammatory cytokines and activation of intracellular signaling pathways sustain the RA symptoms considerably. There is a strong correlation between the expression of cytokines and opioid receptors in the arthritis process. Studies have shown that probiotics via different pathways such as reducing the levels of pro-inflammatory cytokines can alleviate inflammatory symptoms. Therefore, based on the crucial role of cellular and humoral immunity in induction of RA symptoms and potency of probiotics in modulation of immune responses, the purpose of this study was to investigate the effect of orally administered probiotics on the behavioral, cellular and molecular aspects of adjuvant-induced arthritis in male Wistar rats. Methods:Complete Freund’s Adjuvant (CFA)-induced arthritis was caused by single subcutaneous injection of CFA into the rat’s hind paw on day 0. Different doses of probiotics (1/250, 1/500 and 1/1000 [109 CFU/g]) were administered daily (gavage) after CFA injection. Hyperalgesia, edema, serum IL-1β levels, μ-Opioid Receptor (MOR) expression, and p38MAPK (Mitogen-Activated Protein Kinase) activities were assessed on days 0, 7, 14 and 21 of the study. Results: The results of this study indicated the efficacy of probiotics in reducing hyperalgesia, edema, serum levels of Interleukin-1β, and p38MAPK pathway activity during different phases of arthritis as well as increasing the expression of MORs during chronic phase of CFA-induced arthritis. Conclusion: It seems that probiotics can effectively reduce inflammatory symptoms by inhibiting the intracellular signaling pathway and cytokine production.

【 授权许可】

Unknown