Phytochemical Constituents and Biological Activities of Salvia suffruticosa

Research Journal of Pharmacognosy

ARTICLE

Arezoo Rustaie¹  Abbas Hadjiakhoondi¹  Tahmineh Akbarzadeh²  Maliheh Safavi⁴  Nasrin Samadi⁵  Reyhaneh Sabourian²  Mahnaz Khanavi¹ 

[1] Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences;Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences;Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences;Department of Biotechnology, Iranian Research Organization for Science and Technology;Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences;Faculty of Land and Food Systems, University of British Columbia

关键词: Antioxidant;    GC-MS;    Lamiaceae;    MTT assay;    Salvia suffruticosa;   

DOI  :  10.22127/rjp.2018.58491

学科分类：环境科学（综合）

来源: Iranian Society of Pharmacognosy

【 摘 要 】

Background and objectives: Salvia suffruticosa is a perennial plant from Lamiaceae family. Many Salvia species have been employed as medicinal plants; despite the medicinal potentials of S. suffruticosa, there is limited studies regarding its phytochemical profile or biological properties. The aim of the present study was to investigate the chemical constituents of the essential oil and extract of the plant and evaluate its biological activities. Methods: Essential oil from the aerial parts of the plant was extracted by hydrodistillation and analyzed using gas chromatography/mass spectroscopy. Isolation of compounds from methanol and petroleum ether fractions was achieved by using column chromatography with different stationary phases. The structures of the isolated compounds were elucidated by NMR techniques. Cytotoxicity potentials were evaluated using MTT assay and acridine orange/ethidium bromide staining method. Antioxidant activity was assessed by DPPH method. Results: Hydrocarbon sesquiterpenes were identified as the predominant components of the oil, with β-caryophyllene (27.35%), bicyclogermacrene (22.15%), germacrene-D (9.49%) and β-farnesene (9.08%) as the major constituents. Phytochemical analysis of the extract resulted in isolation of lupeol (1), β-sitosterol (2), stigmasterol (3), caffeic acid (4) and 1-feruloyl-β-D-glucopyranose (5). Among the tested samples, lupeol demonstrated the most potent inhibitory activity toward breast cancer cell lines including MCF-7, T-47D and MDA-MB-231 with IC50 values equal to 33.38±2.6, 36.70±3.1 and 23.66±1.4 μg/mL, respectively; caffeic acid with IC50 value of 12.1±1.2 μg/mL showed the most potent radical scavenging activity. Conclusion: The results of this study suggested S. suffruticosa as a promising source of bioactive compounds useful in prevention and treatment of cancer.

【 授权许可】

CC BY-NC