Expression levels of the microRNA maturing microprocessor complex components, Drosha, Dicer, and DGCR8 in PBMCs from ankylosing spondylitis patients

Mediterranean Journal of Rheumatology

ARTICLE

Zeinab Tabrizi¹  Reza Mansouri²  Saeed Aslani¹  Ahmad Reza Jamshidi¹  Mahdi Mahmoudi¹ 

[1] Rheumatology Research Center, Tehran University of Medical Sciences;Immunology Department, Shahid Sadoughi University of Medical Sciences (International Campus)

关键词: Drosha;    Dicer;    DGCR8;    Ankylosing spondylitis;   

DOI  :  10.31138/mjr.28.2.80

学科分类：社会科学、人文和艺术（综合）

来源: PCO Convin S.A.

【 摘 要 】

Objective/Aim: Two major enzymes in the microRNA maturation process, Dicer and Drosha, as well as DGCR8, the assistant of Drosha, function in the microprocessor complex. In this survey, the mRNA expression profiles of Drosha, Dicer, and DGCR8 in peripheral blood mononuclear cells (PBMCs) from ankylosing spondylitis (AS) patients and healthy controls were measured. Methods: Forty patients with AS and 40 age and gender matched healthy individuals were included in the study. PBMCs were separated, total RNA content of the cells was isolated, and first strand cDNA was synthesized. Quantitative analysis was performed through real-time PCR using the SYBR Green gene expression master mix. Results: AS cases expressed the Drosha mRNA almost equal to that of healthy controls (Fold Change= –0.94; P= 0.200). However, both Dicer and DGCR8 mRNA expressions were downregulated in patients relative to healthy subjects (Fold Change= –0.54 and –0.60; P= 0.002 and 0.004, respectively). Conclusion: Our results suggest that downregulation of miRNA maturation components, namely Dicer and DGCR8 may be contributing in the pathogenesis procedure of AS.

【 授权许可】

CC BY-NC