Mutations in Streptomycin Resistance Genes and Their Relationship to Streptomycin Resistance and Lineage of Mycobacterium tuberculosis Thai Isolates

Tuberculosis and Respiratory Diseases

article

Yin Moe Hlaing¹  Pongsri Tongtawe¹  Pramuan Tapchaisri¹  Jeeraphong Thanongsaksrikul¹  Unchana Thawornwan²  Buppa Archanachan¹  Potjanee Srimanote¹ 

[1] Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University;Bamrasnaradura Infectious Diseases Institute

关键词: Mycobacterium tuberculosis;    Streptomycin;    Drug Resistance;    Microbial;    Mutation;    rpsL;    rrs;    gidB;    whiB7;   

DOI  :  10.4046/trd.2017.80.2.159

学科分类：医学（综合）

来源: The Korean Academy of Tuberculosis and Respiratory Diseases

【 摘 要 】

Background Streptomycin (SM) is recommended by the World Health Organization (WHO) as a part of standard regimens for retreating multidrug-resistant tuberculosis (MDR-TB) cases. The incidence of MDR-TB in retreatment cases was 19% in Thailand. To date, information on SM resistance (SMR) gene mutations correlated to the SMR of Mycobacterium tuberculosis Thai isolates is limited. In this study, the mutations in rpsL , rrs , gidB , and whiB7 were investigated and their association to SMR and the lineage of M. tuberculosis were explored. Methods The lineages of 287 M. tuberculosis collected from 2007 to 2011 were identified by spoligotyping. Drug susceptibility profiles were evaluated by the absolute concentration method. Mutations in SMR genes of 46 SM-resistant and 55 SM-susceptible isolates were examined by DNA sequencing. Results Three rpsL (Lys43Arg, Lys88Arg, and Lys88Thr) and two gidB (Trp45Ter and Gly69Asp) mutations were present exclusively in the SM resistant M. tuberculosis . Lys43Arg rpsL was the most predominant SMR mutations (69.6%) and prevailed among Beijing isolates (pC, 615A>G, and 330G>T, as lineage signatures for Beijing and EAI were underscored. This study identified 423G>A gidB as a novel sub-lineage marker for EAI6-BGD1. Conclusion Our study suggested that the majority of SMR in M. tuberculosis Thai isolates were responsible by rpsL and gidB polymorphisms constantly providing the novel lineage specific makers.

【 授权许可】

CC BY-NC