Lung Microbiome Analysis in Steroid-Naїve Asthma Patients by Using Whole Sputum

Tuberculosis and Respiratory Diseases

article

Jae-Woo Jung¹  Jae-Chol Choi¹  Jong-Wook Shin¹  Jae-Yeol Kim¹  In-Won Park¹  Byoung Whui Choi¹  Heung-Woo Park²  Sang-Heon Cho²  Kijeong Kim³  Hye-Ryun Kang² 

[1] Department of Internal Medicine, Chung-Ang University College of Medicine;Department of Internal Medicine, Seoul National University College of Medicine;Department of Microbiology, Chung-Ang University College of Medicine

关键词: Asthma;    Microbiota;    Polymorphism;    Restriction Fragment Length;    RNA;    Ribosomal;    16S;    Sputum;   

DOI  :  10.4046/trd.2016.79.3.165

学科分类：医学（综合）

来源: The Korean Academy of Tuberculosis and Respiratory Diseases

【 摘 要 】

Background Although recent metagenomic approaches have characterized the distinguished microbial compositions in airways of asthmatics, these results did not reach a consensus due to the small sample size, non-standardization of specimens and medication status. We conducted a metagenomics approach by using terminal restriction fragment length polymorphism (T-RFLP) analysis of the induced whole sputum representing both the cellular and fluid phases in a relative large number of steroid naïve asthmatics. Methods Induced whole sputum samples obtained from 36 healthy subjects and 89 steroid-naїve asthma patients were analyzed through T-RFLP analysis. Results In contrast to previous reports about microbiota in the asthmatic airways, the diversity of microbial composition was not significantly different between the controls and asthma patients (p=0.937). In an analysis of similarities, the global R-value showed a statistically significant difference but a very low separation (0.148, p=0.002). The dissimilarity in the bacterial communities between groups was 28.74%, and operational taxonomic units (OTUs) contributing to this difference were as follows: OTU 789 ( Lachnospiraceae ), 517 ( Comamonadaceae , Acetobacteraceae , and Chloroplast ), 633 ( Prevotella ), 645 ( Actinobacteria and Propionibacterium acnes ), 607 ( Lactobacillus buchneri , Lactobacillus otakiensis , Lactobacillus sunkii , and Rhodobacteraceae ), and 661 ( Acinetobacter , Pseudomonas , and Leptotrichiaceae ), and they were significantly more prevalent in the sputum of asthma patients than in the sputum of the controls. Conclusion Before starting anti-asthmatic treatment, the microbiota in the whole sputum of patients with asthma showed a marginal difference from the microbiota in the whole sputum of the controls.

【 授权许可】

CC BY-NC