Impaired Expression of MAPK Is Associated with the Downregulation of TNF-α, IL-6, and IL-10 in Mycobacterium abscessus Lung Disease

Tuberculosis and Respiratory Diseases

article

Yun Su Sim¹  Su-Young Kim¹  Eun Joo Kim¹  Sung Jae Shin²  Won-Jung Koh¹ 

[1] Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine;Department of Microbiology and Research Institute for Medical Sciences, Infection Signaling Network Research Center, Chungnam National University School of Medicine

关键词: Nontuberculous Mycobacteria;    Extracellular Signal-Regulated MAP Kinases;    Mitogen-Activated Protein Kinases;   

DOI  :  10.4046/trd.2012.72.3.275

学科分类：医学（综合）

来源: The Korean Academy of Tuberculosis and Respiratory Diseases

【 摘 要 】

Background Healthy individuals who develop nontuberculous mycobacteria (NTM) lung disease are likely to have specific susceptibility factors which can lead to a NTM infection. The aim of the present study was to investigate the mechanism underlying innate immune responses, including the role of mitogen-activated protein kinase (MAPK), in Mycobacterium abscessus lung disease. Methods Extracellular signal-regulated kinase (ERK1/2) and p38 MAPK expression in monocytes from peripheral blood mononuclear cells were measured by Western blot analysis after stimulation by Mycobacterium avium in five patients with M. abscessus lung disease and seven healthy controls. A M. avium -induced cytokine assay was performed after inhibition of ERK1/2 and p38 MAPK pathways. Results Mycobacterium avium induced p38 and ERK1/2 expression in monocytes from healthy controls and subsequently upregulated tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 production. In monocytes from patients with M. abscessus lung disease, however, induction of p38 and ERK1/2 expression, and the production of TNF-α, IL-6, and IL-10 were significantly lower. Conclusion Decreased activity of MAPK and cytokine secretion in monocytes from patients with M. abscessus lung disease may provide an explanation regarding host susceptibility to these uncommon infections.

【 授权许可】

CC BY-NC