Characterization of Shigella Strains by Plasmid Profile Analysis and Antibiotic Susceptibility Patterns in a Pediatric Hospital in Ahvaz

International Journal of Enteric Pathogens

article

Amin Sakhaei¹  Mohammad Savari²  Leili Shokoohizadeh³  Maryam Hadian⁵  Alireza Ekrami³ 

[1] Department of Microbiology, Karaj Islamic Azad University;Department of Microbiology, School of Medicine, Tehran University of Medical Sciences;Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences;Department of Medical Laboratory Sciences, Ahvaz Jundishapur University of Medical Sciences;Laboratory Department, Abuzar Hospital, Ahvaz Jundishapur University of Medical Sciences

关键词: Dysentery;    Shigellosis;    Extended Spectrum β-Lactamase (ESBL);   

DOI  :  10.17795/ijep29924

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: High incidences of dysentery and diarrhea were reported in a pediatric hospital in Ahvaz, Iran during March to April, 2013. Objectives: A cross-sectional study was therefore undertaken to identify the causative agents. Patients and Methods: A total of 230 diarrhea samples were collected from the patients and analyzed by routine bacteriological methods. Bacterial identification, serological assay, antimicrobial susceptibility testing, extended spectrum β-lactamases (ESBLs) screening and plasmid profile analysis were performed according to the standard guidelines. Results: A total of 70 Shigella strains including %70 (n = 49) S. sonnei and 30% (n = 21) S. flexneri were isolated from diarrhea samples. Most of the Shigella isolates showed high degrees of resistance to ampicillin, ulafamethoxazole- trimethoprime and cefexim. Concurrent resistance to sulafametoxazole- trimethoprime and ampicillin was the most common resistance pattern. Overall, 11.4% of Shigella isolates showed the ESBL producer criteria. The plasmid profile patterns of all the strains were determined by a modified alkaline lysis method. By plasmid profile analysis 23 genotypes were identified among all the isolates, 14 and 9 genotypes among the S. sonnei and S. flexneri respectively. S. sonnei and S. flexneri isolates demonstrated unique plasmid profiles. Conclusions: These data demonstrated that S. sonnei strains are the main cause of shigellosis as the prevalent Shigella serotype in Iran. We also found that the antibiotic resistance rates are increasing among Shigella strains. Plasmid profile analysis is more reliable than antibiotic susceptibility patterns in epidemiologic studies.

【 授权许可】

CC BY-NC