Probiotic Therapy, What is the most Effective Method for Host Protection Against Enteric Pathogen

International Journal of Enteric Pathogens

article

Sayyed Mohammad Hossein Ghaderian¹  Mahboobeh Mehrabani Natanzi²  Mahdi Goudarzvand³  Zohreh Khodaii² 

[1] Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences;Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences;Physiology and Pharmacology Department, Faculty of Medicine, Alborz University of Medical Sciences

关键词: Probiotics;    Pathogen Exclusion;    Co-Incubation;    Pre-Incubation;    Caco-2 Cells;   

DOI  :  10.17795/ijep13289

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: Prevention of adverse microbial colonization is supposed to be the most important beneficial effect of the gut microflora. Objectives: The aims of the present study were to compare the effect of co-incubation, pre-incubation and supernatant of sixteen probiotic strains on prevention of enteroinvasive E. coli adhesion. Materials and Methods: Probiotic strains were added to Caco-2 cells followed by E. coli in pre-incubation assay. Tested strains and enteroinvasive E. coli were added to cell lines at the same time in co-incubation assay. Finally, enteroinvasive E. coli was treated with bacteria free supernatant of test strains then added to cell line in treatment with bacteria free supernatant assay. Results: This study showed that the most effective assays in prevention of pathogen adherence were treatment with bacteria free supernatant and pre-incubation respectively. Conclusions: The effect of probiotic bacteria by-products on pathogen exclusion may be of more importance in protecting the host. Therefore, gut colonization or at least persistent presence of probiotics may be helpful in infection prevention.

【 授权许可】

CC BY-NC