Antibiotic Susceptibility of Helicobacter pylori Clinical Isolates in Hamadan, West of Iran

International Journal of Enteric Pathogens

article

Amir Majlesi¹  Masood Sayedin Khorasani²  Ali Reza Khalilian¹  Mohammad Mehdi Aslani³  Mohammad Jaefari⁴  Mohammad Yousef Alikhani² 

[1] Department of Gastroenterology, Faculty of Medicine, Hamadan University of Medical Sciences;Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences;Department of Microbiology, Pasteur Institute of Iran;Department of Pathology, Faculty of Medicine, Hamadan University of Medical Sciences

关键词: Helicobacter pylori;    Drug Resistance;    Bacterial;    Metronidazole;    Clarithromycin;   

DOI  :  10.17795/ijep9344

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: Surveillance data on Helicobacter pylori antibiotic susceptibilities are limited in Hamadan, Iran. Since antibiotic resistance is one of the reasons in therapies failure. Objectives: Thus the resistance patterns of H. pylori strains to the antibiotics metronidazole, clarithromycin, amoxicillin and tetracycline were evaluated. Patients and Methods: Gastric biopsy specimens of 153 patients with nonulcer dyspepsia, peptic ulcer dyspepsia, and peptic cancer collected during May 2010 to February of 2011, and were cultured on Brucella agar (Merck, Germany) under microaerophilic conditions. H. pylori isolates were identified using standard biochemical test. Eighty three (54.2 %) specimens had positive results by culture. Antimicrobial susceptibility testing was performed by disc diffusion method. Results: Totally in vitro resistance rates were 63.8% for metronidazole, 26.5% for clarithromycin, and 7.2% for amoxicillin. Although, 25.3% of strains showed resistance to two antibiotics, and 3.6% to three antibiotics. Tetracycline resistance was identified in only two isolates. Fifty nine percent of the clarithromycin resistant strains also showed resistance to metronidazole. No gender and age associations with resistance were detected. Conclusion: Our results showed a high incidence of metronidazole resistance (often combined with clarithromycin-resistance) in the isolates. Continuous surveillance is recommended to examine the treatment strategies for H. pylori eradication.

【 授权许可】

CC BY-NC