Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens

Journal of Veterinary Medical Research

article

H. Hassan, Walid¹  H. Abed, Ahmed¹  Thabet, Abd El Rady²  A.M. El Nady, Eman² 

[1] Department of Bacteriology, Faculty of Veterinary Medicine, Beni-Suef University.;Department of Bacteriology, Animal Health Research Institute, Assiut Branch.

关键词: multidrug resistance;    Broiler Chicken;    Salmonellae;    β;    lactamases;    stn;    invA;    hilA;   

DOI  :  10.21608/jvmr.2018.43309

学科分类：社会科学、人文和艺术（综合）

来源: Beni-Suef University

【 摘 要 】

Salmonellosis is a major problem for the poultry industry, and this problem represents a critical food safety hazard. Resistance to antimicrobial agents within nontyphoidal Salmonellae is a serious problem. The present study aimed to analyze genetically some β-lactamase resistance genes and some virulence associated genes in Salmonella isolates from broiler chicken. Five hundred samples were collected from diseased broiler chickens of different ages (3-6 weeks) from different farms in Assiut Governorate in Egypt during the period from January 2015 to December 2015. Bacteriological examination showed that 26 Salmonella isolates were recovered with a prevalence rate of 5.2% Serotyping of Salmonella isolates showed that S. Enteritidis S. Typhimurium, and S. Kentuky were identified at rates of 50%, 30.8% and 19.2%, respectively. Results of antibiogram showed that 18 Salmonella isolates (92.3%) were multidrug resistant. All isolates were screened for the presence of 2 β-lactamase resistance genes (blaCTX and blaCMY) as well as 3 virulence genes (stn, invA and hilA) using multiplex PCR. The overall prevalences were 53.9% for blaCTX and 34.6% for blaCMY. Meanwhile, stn, invA and hilA genes were found in 96.2%, 100% and 84.7% of isolates, respectively.

【 授权许可】

CC BY