Age-related rhesus macaque models of COVID-19

Animal Models and Experimental Medicine

article

Pin Yu¹  Feifei Qi¹  Yanfeng Xu¹  Fengdi Li¹  Peipei Liu²  Jiayi Liu³  Linlin Bao¹  Wei Deng¹  Hong Gao¹  Zhiguang Xiang¹  Chong Xiao¹  Qi Lv¹  Shuran Gong¹  Jiangning Liu¹  Zhiqi Song¹  Yajin Qu¹  Jing Xue¹  Qiang Wei¹  Mingya Liu¹  Guanpeng Wang¹  Shunyi Wang¹  Haisheng Yu¹  Xing Liu¹  Baoying Huang²  Wenling Wang²  Li Zhao²  Huijuan Wang²  Fei Ye²  Weimin Zhou²  Wei Zhen²  Jun Han²  Guizhen Wu²  Qi Jin⁴  Jianwei Wang⁵  Wenjie Tan⁶  Chuan Qin¹ 

[1] Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College;MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention;Department of Radiology, Bejing Anzhen Hospital, Capital Medical University;Institute of Pathogen Biology, Chinese Academy of Medical Sciences;Chinese Academy of Medical Sciences and Peking Union Medical College;MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC

关键词: pathogenicity;    pneumonia;    rhesus macaque model;    SARS-CoV-2;   

DOI  :  10.1002/ame2.12108

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Background: Since December 2019, an outbreak of the Corona Virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, has become a public health emergency of international concern. The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models. Methods: Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2, and then analyzed by clinical signs, viral replication, chest X-ray, histopathological changes and immune response. Results: Viral replication of nasopharyngeal swabs, anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge. Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema, notably, old monkeys exhibited diffuse severe interstitial pneumonia. Viral antigens were detected mainly in alveolar epithelial cells and macrophages. Conclusion: SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys. Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection.

【 授权许可】

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