Lgr5 + intestinal stem cell sorting and organoid culture

Animal Models and Experimental Medicine

article

Ruixue Liu¹  Haifeng Li¹  Juan Cai¹  Qiang Wei¹  Xiaonan Han¹ 

[1] Key Laboratory of Human Disease Comparative Medicine, The Ministry of Health, Institute of Laboratory Animal Science (ILAS), Chinese Academy of Medical Science (CAMS) and Peking Union Medical College (PUMC)

关键词: colonoids;    enteroids;    Lgr5 intestinal stem cells;    organoid culture;   

DOI  :  10.1002/ame2.12057

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Intestinal epithelial stem cells (IESCs) are one of the most rapidly self‐renewing and proliferating adult stem cells. The IESCs reside at the bottom of intestinal and colonic crypts, giving rise to all intestinal epithelial lineages and maintaining intestinal epithe‐ lial replenishment. The technique of three‐dimensional culture based upon intestinal stem cell biology has been recently developed to study gastrointestinal development and disease pathogenesis. Here, we summarize the techniques used to isolate Lgr5‐ positive IESCs to form the enteroids from intestine or colonoids from colon, and pre‐ sent the means to examine these organoid functions. This study will provide a simple and practical way for producing intestinal tissues in the laboratory.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND