HoxB8 neutrophils replicate Fcγ receptor and integrin-induced neutrophil signaling and functions

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

article

Julia Y. Chu¹  Barry McCormick¹  Greta Mazelyte¹  Melina Michael¹  Sonja Vermeren¹ 

[1] The MRC Centre for Inflammation Research, University of Edinburgh

关键词: granulocytes;    human cell lines;    monocytes/macrophages;    neutrophils;    adhesion molecules;    intracellular signaling;    Fc Receptors;    transgenic/knockout mice;   

DOI  :  10.1002/JLB.1AB0618-232R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Neutrophils are short-lived, terminally differentiated leukocytes that form an essential part of host immunity and play a key role in acute and chronic inflammation. The analysis of these important cells is hindered by the fact that neutrophils are not amenable to culture, transfection, or transduction. Conditionally HoxB8-immortalized mouse hematopoietic progenitors are suitable for in vitro differentiation of a range of myeloid cells, including neutrophils. Integrins and Fc?Rs are cell surface receptors, the ligation of which is required for a range of neutrophil functions that are important in health and disease. We show here that HoxB8 neutrophils express major neutrophil integrins and Fc?Rs. They respond to Fc?R and integrin stimulation in a manner that is comparable with primary neutrophils, in terms of intracellular signaling. HoxB8 neutrophils also perform a range of Fc?R/integrin-dependent neutrophil functions, including, generation of reactive oxygen species, degranulation, and chemotaxis. Our findings suggest that HoxB8 neutrophils represent a faithful experimental model system for the analysis of Fc and integrin receptordependent neutrophil functions.

【 授权许可】

CC BY