Transcriptional profiling of eosinophil subsets in interleukin-5 transgenic mice

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

article

Kirsten A. Fairfax¹  Jessica E. Bolden¹  Aaron J. Robinson¹  Erin C. Lucas¹  Tracey M. Baldwin¹  Kerry A. Ramsay¹  Rebecca Cole¹  Douglas J. Hilton¹  Carolyn A. de Graaf¹ 

[1] Division of Molecular Medicine, Walter and Eliza Hall Institute of Medical Research;Department of Medical Biology, The University of Melbourne

关键词: gene expression;    granulocyte;    progenitor;    Siglec-F;    trajectory;   

DOI  :  10.1002/JLB.6MA1117-451R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Eosinophils are important in fighting parasitic infections and are implicated in the pathogenesis of asthma and allergy. IL-5 is a critical regulator of eosinophil development, controlling proliferation, differentiation, and maturation of the lineage. Mice that constitutively express IL-5 have in excess of 10-fold more eosinophils in the hematopoietic organs than their wild type (WT) counterparts. We have identified that much of this expansion is in a population of Siglec-F high eosinophils, which are rare inWT mice. In this study, we assessed transcription in myeloid progenitors, eosinophil precursors, and Siglec-F medium and Siglec-F high eosinophils from IL-5 transgenic mice and in doing so have created a useful resource for eosinophil biologists. We have then utilized these populations to construct an eosinophil trajectory based on gene expression and to identify gene sets that are associated with eosinophil lineage progression. Cell cycle genes were significantly associated with the trajectory, and we experimentally demonstrate an increasing trend toward quiescence along the trajectory. Additionally, we found gene expression changes associated with constitutive IL-5 signaling in eosinophil progenitors, many of which were not observed in eosinophils.

【 授权许可】

CC BY