Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
Galectin-3 type-C self-association on neutrophil surfaces, The carbohydrate recognition domain regulates cell function | |
article | |
Martina Sundqvist1  Amanda Welin1  Jonas Elmwall1  Veronica Osla1  Ulf J. Nilsson2  Hakon Leffler3  Johan Bylund4  Anna Karlsson1  | |
[1] Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at the University of Gothenburg;Centre for Analysis and Synthesis, Department of Chemistry, Lund University;Department of Laboratory Medicine, Section of Microbiology, Lund University;Department of Oral Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg | |
关键词: carbohydrate recognition domain; galectin; neutrophils; priming; ROS production; | |
DOI : 10.1002/JLB.3A0317-110R | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
Galectin-3 is an endogenous ?-galactoside-binding lectin comprising a carbohydrate recognition domain (CRD) linked to a collagen-like N-domain. Both domains are required for galectin-3 to induce cellular effects; a C-terminal fragment of galectin-3, galectin-3C, containing the CRD but lacking the N-domain, binds cell surface glycoconjugates but does not induce cellular effects since cross-linking promoted by the N-domain is thought to be required. Instead, galectin-3C is proposed to antagonize the effects of galectin-3 by competing for binding sites. The aim of this study was to investigate the effects of galectin-3C on galectin-3 interactions with human neutrophils. Recombinant galectin-3C inhibited galectin-3-induced production of reactive oxygen species in primed neutrophils. Surprisingly, this inhibition was not due to competitive inhibition of galectin-3 binding to the cells. In contrast, galectin-3C potentiated galectin-3 binding, in line with emerging evidence that galectin-3 can aggregate not only through the N-domain but also through the CRD. The cell surface interaction between galectin-3C and galectin-3 was corroborated by colocalization of fluorescently labeled galectin-3 and galectin-3C. Galectin-3C can be generated in vivo through cleavage of galectin-3 by proteases. Indeed, in circulation, galectin-3 and galectin3C were both attached to the cell surface of neutrophils, which displayed great capacity to bind additional galectin-3 and galectin-3C. In conclusion, galectin-3C enhances galectin-3 binding to neutrophils by nonactivating type-C self-association, in parallel to inhibiting neutrophil activation by galectin-3 (induced by type-N self-association). This implicates type-C self-association as a termination system for galectin-3-induced cell activation, with the purpose of avoiding oxidantdependent tissue damage.
【 授权许可】
CC BY
【 预 览 】
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RO202105310000609ZK.pdf | 472KB | download |