Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta

FEBS Letters

article

Mushtaq M. Khazeem¹  Ian G. Cowell¹  Lauren F. Harkin¹  John W. Casement²  Caroline A. Austin¹ 

[1] Faculty of Medical Sciences, Biosciences Institute, Newcastle University;Bioinformatics Support Unit, Faculty of Medical Sciences, Newcastle University

关键词: carbonyl reductase 1;    cardiotoxicity;    CBR1;    DNA topoisomerase;    TOP2B;    topologically associating domains;   

DOI  :  10.1002/1873-3468.13904

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA topoisomerase II beta (TOP2B) has a role in transcriptional regulation. Here, to further investigate transcriptional regulation by TOP2B, we used RNA-sequencing and real-time PCR to analyse the differential gene expression profiles of wild-type and two independent TOP2B-null pre-B Nalm-6 cell lines, one generated by targeted insertion and the other using CRISPR-Cas9 gene editing. We identified carbonyl reductase 1 (CBR1) among the most significantly downregulated genes in these TOP2B-null cells. Reduced CBR1 expression was accompanied by loss of binding of the transcription factors USF2 and MAX to the CBR1 promoter. We describe possible mechanisms by which loss of TOP2B results in CBR1 downregulation. To our knowledge, this is the first report of a link between TOP2B and CBR1.

【 授权许可】

Unknown