Imprinted X-chromosome inactivation impacts primitive endoderm differentiation in mouse blastocysts

FEBS Letters

article

Atsushi Fukuda¹  Nami Motosugi¹  Mikiko Ando¹  Minoru Kimura³  Akihiro Umezawa²  Hidenori Akutsu² 

[1] Medical Division, Institute of Innovative Science and Technology, Tokai University;Center for Regenerative Medicine, National Center for Child Health and Development;Institute of Medical Sciences, Tokai University

关键词: imprinted X-chromosome inactivation;    lineage specification;    sex difference;   

DOI  :  10.1002/1873-3468.13676

来源: John Wiley & Sons Ltd.

【 摘 要 】

Epigenetic and transcriptome alterations are essential for lineage specification, represented by imprinted X-chromosome inactivation (iXCI) in female mouse preimplantation embryos. However, how various factors affect transcriptome states and lineage commitment remains unclear. We found that in vitro culture duration strongly influences transcriptional variation compared to iXCI loss. Single-cell analysis of the inner cell mass (ICM) for major transcription and epigenomic factors revealed that sex-specific differences in expression are diminished by loss of iXCI in the primitive endoderm (PrE) but not in the epiblast. Females had a higher proportion of ICM compared to that in males, and PrE development was affected by iXCI states in female embryos. Our findings provide insight into sex differences and iXCI function in lineage specification.

【 授权许可】

Unknown