Resolution of ROS-induced G-quadruplexes and R-loops at transcriptionally active sites is dependent on BLM helicase

FEBS Letters

article

Jun Tan¹  Xiangyu Wang¹  Laiyee Phoon¹  Haibo Yang¹  Li Lan¹ 

[1] Massachusetts General Hospital Cancer Center, Harvard Medical School;Department of Radiation Oncology, Harvard Medical School, Massachusetts General Hospital

关键词: BLM helicase;    DNA damage repair;    G-quadruplex;    R-loop;    ROS;    transcription;   

DOI  :  10.1002/1873-3468.13738

来源: John Wiley & Sons Ltd.

【 摘 要 】

R-loops and G-quadruplexes (G4s) are noncanonical secondary DNA structures. Here, we show that reactive oxygen species (ROS) induce G4 formation as well as R-loops at transcriptionally active sites. Importantly, the G4 structure is subsequently triggered by R-loop formation after damage. Once G4 is formed within an R-loop, it reversely stabilizes the R-loop. Notably, the helicase activity of Bloom syndrome protein is essential for the resolution of both R-loops and G4s. Unresolved G4s and R-loops delay the repair of ROS-induced damage at actively transcribed sites. Together, our results demonstrate that interregulation between R-loops and G4s induced by ROS is essential for repair at transcriptionally active sites.

【 授权许可】

Unknown