Getting out of mitosis: spatial and temporal control of mitotic exit and cytokinesis by PP1 and PP2A

FEBS Letters

article

James Holder¹  Elena Poser¹  Francis A. Barr¹ 

[1]Department of Biochemistry, University of Oxford

关键词: cell cycle;    kinase;    mitosis;    mitotic exit;    phosphatase;    proteolysis;   

DOI  :  10.1002/1873-3468.13595

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here, we will review the evidence showing that mitotic exit is initiated by regulated proteolysis and then driven by the PPP family of phosphoserine/threonine phosphatases. Rapid APC/CCDC20 and ubiquitin-dependent proteolysis of cyclin B and securin initiates sister chromatid separation, the first step of mitotic exit. Because proteolysis of Aurora and Polo family kinases dependent on APC/CCDH1 is relatively slow, this creates a new regulatory state, anaphase, different to G2 and M-phase. We will discuss how the CDK1-counteracting phosphatases PP1 and PP2A-B55, together with Aurora and Polo kinases, contribute to the temporal regulation and order of events in the different stages of mitotic exit from anaphase to cytokinesis. For PP2A-B55, these timing properties are created by the ENSA-dependent inhibitory pathway and differential recognition of phosphoserine and phosphothreonine. Finally, we will discuss how Aurora B and PP2A-B56 are needed for the spatial regulation of anaphase spindle formation and how APC/C-dependent destruction of PLK1 acts as a timer for abscission, the final event of cytokinesis.

【 授权许可】

Unknown