PES1 is regulated by CD44 in liver cancer stem cells via miR-105-5p

FEBS Letters

article

Shiruo Wei¹  Kaiyu Liu²  Qihua He¹  Yi Gao²  Li Shen¹ 

[1]Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center

[2]Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University

关键词: C3A cells;    CD44;    induced cancer stem cells;    miR-105-5p;    PES1;   

DOI  :  10.1002/1873-3468.13459

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pescadillo (PES1) is a key molecule for ribosome formation in mammalian cells. In this study, human hepatoma C3A cells were reprogrammed by four transcription factors, Oct4, Sox2, Klf4 and c-Myc, into induced cancer stem cells, termed C3A-induced cancer stem cells (C3A-iCSCs). We found that PES1 was up-regulated in C3A-iCSCs and promoted cell proliferation. Moreover, the cancer stem cell marker CD44, which is located in the cytomembrane, translocated to the nucleus and was up-regulated in C3A-iCSCs. Our results suggest that CD44 has a negative effect on miR-105-5p. We found that PES1 is a direct target of, and was negatively regulated by, miR-105-5p. In summary, CD44 regulates PES1 in liver cancer stem cells via miR-105-5p to promote cell growth.

【 授权许可】

Unknown