Mechanisms and biomedical implications of –1 programmed ribosome frameshifting on viral and bacterial mRNAs

FEBS Letters

article

Natalia Korniy¹  Ekaterina Samatova¹  Maria M. Anokhina²  Frank Peske¹  Marina V. Rodnina¹ 

[1] Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry;Institute of Pathology, University Hospital of Cologne

关键词: frameshifting;    protein synthesis;    recoding;    regulation;    ribosome;    RNA;    translation;    tRNA;   

DOI  :  10.1002/1873-3468.13478

来源: John Wiley & Sons Ltd.

【 摘 要 】

Some proteins are expressed as a result of a ribosome frameshifting event that is facilitated by a slippery site and downstream secondary structure elements in the mRNA. This review summarizes recent progress in understanding mechanisms of –1 frameshifting in several viral genes, including IBV 1a/ 1b, HIV-1 gag-pol, and SFV 6K, and in Escherichia coli dnaX. The exact frameshifting route depends on the availability of aminoacyl-tRNAs: the ribosome normally slips into the –1-frame during tRNA translocation, but can also frameshift during decoding at condition when aminoacyl-tRNA is in limited supply. Different frameshifting routes and additional slippery sites allow viruses to maintain a constant production of their key proteins. The emerging idea that tRNA pools are important for frameshifting provides new direction for developing antiviral therapies.

【 授权许可】

Unknown