Ribosomal mutation in helix 32 of 18S rRNA alters fidelity of eukaryotic translation start site selection

FEBS Letters

article

Charles Antony A¹  Anup Kumar Ram¹  Kalloly Dutta¹  Pankaj V. Alone¹ 

[1] School of Biological Sciences, National Institute of Science Education and Research;Homi Bhabha National Institute (HBNI)

关键词: eIF5;    Gcd- phenotype;    Gcn- phenotype;    ribosome;    Sui- phenotype;   

DOI  :  10.1002/1873-3468.13369

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 40S ribosome plays a critical role in start codon selection. To gain insights into the role of its 18S rRNA in start codon selection, a suppressor screen was performed that suppressed the preferential UUG start codon recognition (Suppressor of initiation codon: Sui¯ phenotype) associated with the eIF5G31R mutant. The C1209U mutation in helix h32 of 18S rRNA was found to suppress the Sui¯ and Gcn¯ (failure to derepress GCN4 expression) phenotype of the eIF5G31R mutant. The C1209U mutation suppressed Sui¯ and Gcd¯ (constitutive derepression of GCN4 expression) phenotype of eIF2bS264Y, eIF1K60E, and eIF1A-DC mutation. We propose that the C1209U mutation in 40S ribosomal may perturb the premature head rotation in ‘Closed/PIN’ state and enhance the stringency of translation start site selection.

【 授权许可】

Unknown