TRB1 negatively regulates gluconeogenesis by suppressing the transcriptional activity of FOXO1

FEBS Letters

article

Kaori Tsuzuki¹  Yuka Itoh¹  Yasumichi Inoue¹  Hidetoshi Hayashi¹ 

[1] Department of Cell Signaling, Graduate School of Pharmaceutical Sciences, Nagoya City University;Department of Biochemistry, Graduate School of Medicine, University of Yamanashi;Department of Innovative Therapeutics Sciences, Cooperative Major in Nanopharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University

关键词: acetylation;    CBP;    FOXO1;    glucose metabolism;    TRB1;   

DOI  :  10.1002/1873-3468.13314

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tribbles related homolog 1 is the mammalian ortholog of Tribbles, which controls cell division and migration during development in Drosophila. TRB1 is a pseudokinase and functions as a scaffold protein. Recent findings suggest that TRB1 plays important roles in hepatic lipid metabolism and participates in insulin resistance. However, the underlying mechanisms have not yet been elucidated. Here, we demonstrate that TRB1 suppresses FOXO1 transcriptional activity to downregulate the expression of G6Pase and PEPCK, which encode gluconeogenic rate-limiting enzymes. TRB1 knockdown enhances FOXO1 binding to the gluconeogenic gene promoters. It also increases FOXO1 acetylation and recruits CBP to the binding sequence of FOXO1. These results suggest that TRB1 suppresses the expression of G6Pase and PEPCK by attenuating FOXO1 transcriptional activity and negatively regulates gluconeogenesis.

【 授权许可】

Unknown