Time-resolved interaction proteomics of the GIGANTEA protein under diurnal cycles in Arabidopsis

FEBS Letters

article

Johanna Krahmer¹  Greg S. Goralogia³  Akane Kubota³  Argyris Zardilis¹  Richard S. Johnson⁵  Young Hun Song³  Michael J. MacCoss⁵  Thierry Le Bihan¹  Karen J. Halliday²  Takato Imaizumi³  Andrew J. Millar¹ 

[1] SynthSys and School of Biological Sciences, University of Edinburgh;Institute of Molecular Plant Sciences, University of Edinburgh;Department of Biology, University of Washington;Graduate School of Biological Sciences, Nara Institute of Science and Technology;Department of Genome Sciences, University of Washington;Department of Life Sciences, Ajou University

关键词: affinity purification;    Arabidopsis thaliana;    circadian rhythms;    flowering time;    quantitative mass spectrometry;   

DOI  :  10.1002/1873-3468.13311

来源: John Wiley & Sons Ltd.

【 摘 要 】

The plant-specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post-translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI’s multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time-specific manner, using proteomics on Arabidopsis plants expressing epitope-tagged GI. We detected previously identified (in)direct interactors of GI, as well as proteins implicated in protein folding, or degradation, and a previously uncharacterized transcription factor, CYCLING DOF FACTOR6 (CDF6 ). We verified CDF6’s direct interaction with GI, and ZEITLUPE/FLAVIN-BINDING, KELCH REPEAT, F-BOX 1/LIGHT KELCH PROTEIN 2 proteins, and demonstrated its involvement in photoperiodic flowering. Extending interaction proteomics to time series provides a data resource of candidate protein targets for GI’s post-translational control.

【 授权许可】

Unknown