FEBS Letters | |
Time-resolved interaction proteomics of the GIGANTEA protein under diurnal cycles in Arabidopsis | |
article | |
Johanna Krahmer1  Greg S. Goralogia3  Akane Kubota3  Argyris Zardilis1  Richard S. Johnson5  Young Hun Song3  Michael J. MacCoss5  Thierry Le Bihan1  Karen J. Halliday2  Takato Imaizumi3  Andrew J. Millar1  | |
[1] SynthSys and School of Biological Sciences, University of Edinburgh;Institute of Molecular Plant Sciences, University of Edinburgh;Department of Biology, University of Washington;Graduate School of Biological Sciences, Nara Institute of Science and Technology;Department of Genome Sciences, University of Washington;Department of Life Sciences, Ajou University | |
关键词: affinity purification; Arabidopsis thaliana; circadian rhythms; flowering time; quantitative mass spectrometry; | |
DOI : 10.1002/1873-3468.13311 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The plant-specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post-translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI’s multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time-specific manner, using proteomics on Arabidopsis plants expressing epitope-tagged GI. We detected previously identified (in)direct interactors of GI, as well as proteins implicated in protein folding, or degradation, and a previously uncharacterized transcription factor, CYCLING DOF FACTOR6 (CDF6 ). We verified CDF6’s direct interaction with GI, and ZEITLUPE/FLAVIN-BINDING, KELCH REPEAT, F-BOX 1/LIGHT KELCH PROTEIN 2 proteins, and demonstrated its involvement in photoperiodic flowering. Extending interaction proteomics to time series provides a data resource of candidate protein targets for GI’s post-translational control.
【 授权许可】
Unknown
【 预 览 】
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