Molecular Identification of Extended-Spectrum β-lactamase and Integron Genes in Klebsiella pneumonia

Journal of Nepal Medical Association

article

Ehsan Estabraghi¹  Taghi Zahraei Salehi²  Kumarss Amini³  Mahmoud Jamshidian⁴ 

[1] Department of Microbiology, Science and Research Branch, Islamic Azad University of Science and Research of Tehran;Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran;Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University;Department of Microbiology, Science and Research Branch, Islamic Azad University

关键词: fKlebsiella pneumonia;    integrons;    drug resistance.;   

DOI  :  10.31729/jnma.2822

学科分类：社会科学、人文和艺术（综合）

来源: Nepal Medical Association since

【 摘 要 】

Introduction: Infections caused by Gram negative bacteria, producing extended-spectrum β-lactamase, including Klebsiella pneumoniae are increasing all over the world with high morbidity and mortality. The aim of the present study was determined antimicrobial profile susceptibility and the prevalence of antibiotic resistance genes by multiplex PCR. Methods: In the present study, we obtained one-hundred isolates of K. pneumoniae from different clinical samples. The antibiotic susceptibility testing was done in thirteen antibiotic and, therefore, M-PCRs were conducted using the DNA amplification for detection of ESBLs (blaTEM, blaCTX-M, blaSHV) and int (I, II, III) genes. Results: The results of resistance to amoxicillin/clavulanate, ciprofloxacin, amikacin, trimethoprim-sulfamethoxazole, cefotaxime, ampicillin, aztreonam, imipenem, gentamicin, ceftazidime, Cefepime, ceftriaxone and levofloxacin were obtained 37%, 37%, 93%, 84%, 52%, 87%, 59%, 8%, 24%, 67%, 52%, 43% and 26%, respectively. The frequency of the extended-spectrum β-lactamase K. pneumoniae was obtained 37%. The prevalence of resistance genes of ESBLs in the M-PCR method showed that the blaTEM, blaCTX and blaSHV were 38%, 24% and 19%, respectively, however, only 8 (8%) out of 100 isolates were found to have positive outcomes for the existence of class 1 integrons and there were no detected class 2 or class 3 integrons. Conclusions: Our results recommend the likely co-carriage of some ESBLs genes and antibiotic resistance integrons on the same plasmids harboring the MDR genes.

【 授权许可】

CC BY