Revealing oral microbiota composition and functionality associated with heavy cigarette smoking

Journal of Translational Medicine

Nihar Ranjan Dash¹  Dua’a Mohammad Hasan Alnusairat¹  Islam Mohammad Ismail Darwish¹  Mohammed Elkhazendar¹  Mohammad Tahseen Al Bataineh²  Mohamed Saleh Al-Hajjaj³  Qutayba Hamid⁴ 

[1] Clinical Sciences Department, College of Medicine, University of Sharjah, 27272, Sharjah, United Arab Emirates;Clinical Sciences Department, College of Medicine, University of Sharjah, 27272, Sharjah, United Arab Emirates;Sharjah Institute for Medical Research, University of Sharjah, Sharjah, United Arab Emirates;Clinical Sciences Department, College of Medicine, University of Sharjah, 27272, Sharjah, United Arab Emirates;University Hospital Sharjah, Sharjah, United Arab Emirates;Sharjah Institute for Medical Research, University of Sharjah, Sharjah, United Arab Emirates;Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada;

关键词: Fagerström test;    Nicotine dependence;    Oral microbiota;    Shotgun metagenomics;   

DOI  :  10.1186/s12967-020-02579-3

来源: Springer

【 摘 要 】

BackgroundHeavy tobacco smoking, a hallmark feature of lung cancer, is drastically predominant in Middle Eastern populations. The precise links between nicotine dependence and the functional contribution of the oral microbiota remain unknown in these populations.MethodsWe evaluated the composition and functional capabilities of oral microbiota with relation to cigarette smoking in 105 adults through shotgun metagenomics using buccal swabs.ResultsThe oral microbiota composition in our study subjects was dominated by the phyla Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes, in addition to the genera Prevotella and Veillonella, similar to previously described westernized cohorts. Furthermore, the smoker's oral microbiota represented a significant abundance of Veillonella dispar, Leptotrichia spp. and Prevotella pleuritidis when compared to non-smokers. Within the smoking groups, differential relative abundance testing unveiled relative abundance of Streptobacillus hongkongensis, Fusobacterium massiliense, Prevotella bivia in high nicotine dependent compared to low nicotine dependent profiles based on Fagerström Test for Nicotine Dependence. Functional profiling showed marked differences between smokers and non-smokers. Smokers exhibited an enrichment of Tricarballylate utilization and Lactate racemization when compared to the non-smokers. According to their nicotine dependence, enrichment of Xanthosine utilization, p-Aminobenzoyl-Glutamate utilization, and multidrug efflux pump in Campylobacter jejuni biosynthesis modules were detected in the high nicotine dependent group.ConclusionsThese compositional and functional differences may provide critical insight on how variations in the oral microbiota could predispose to respiratory illnesses and smoke cessation relapse in cigarette smokers. In particular, the observed enrichment of Fusobacterium and Prevotella in the oral microbiota possibly suggests an intriguing linkage to gut and lung cancers.

【 授权许可】

CC BY