A biomedical Engineering Laboratory module for exploring involuntary muscle reflexes using Electromyography

Journal of Biological Engineering

Karly S. Franz¹  Dawn M. Kilkenny²  Kramay Patel³ 

[1] Institute of Biomedical Engineering, University of Toronto, 164 College St Room 407, M5S 3G9, Toronto, ON, Canada;Bloorview Research Institute, Holland Bloorview Kids Rehabilitation, 150 Kilgour Rd, M4G 1R8, East York, ON, Canada;Institute of Biomedical Engineering, University of Toronto, 164 College St Room 407, M5S 3G9, Toronto, ON, Canada;Institute for Studies in Transdisciplinary Engineering Education & Practice, University of Toronto, 35 St. George Street, M5S 1A4, Toronto, ON, Canada;Institute of Biomedical Engineering, University of Toronto, 164 College St Room 407, M5S 3G9, Toronto, ON, Canada;Krembil Research Institute, 60 Leonard Avenue, M5T 0S8, Toronto, ON, Canada;Faculty of Medicine, University of Toronto, 1 King’s College Circle, M5S 1A8, Toronto, ON, Canada;

关键词: Electromyography;    Biomedical engineering;    Undergraduate physiology;    Laboratory protocol;    TENS;    Electrical stimulation;    Stretch reflex;   

DOI  :  10.1186/s13036-020-00248-z

来源: Springer

【 摘 要 】

BackgroundUndergraduate biomedical engineering (BME) students interested in pursuing a career in research and development of medical or physiological monitoring devices require a strong foundation in biosignal analysis as well as physiological theory. Applied learning approaches are reported to be effective for reinforcing physiological coursework; therefore, we propose a new laboratory protocol for BME undergraduate physiology courses that integrates both neural engineering and physiological concepts to explore involuntary skeletal muscle reflexes. The protocol consists of two sections: the first focuses on recruiting soleus motor units through transcutaneous electrical nerve stimulation (TENS), while the second focuses on exploring the natural stretch reflex with and without the Jendrassik maneuver. In this case study, third-year biomedical engineering students collected electromyographic (EMG) activity of skeletal muscle contractions in response to peripheral nerve stimulation using a BioRadio Wireless Physiology Monitor system and analyzed the corresponding signal parameters (latency and amplitude) using the MATLAB platform.Results/protocol validationElectrical tibial nerve stimulation successfully recruited M-waves in all 8 student participants and F-waves in three student participants. The students used this data to learn about orthodromic and antidromic motor fiber activation as well as estimate the neural response latency and amplitude. With the stretch reflex, students were able to collect distinct signals corresponding to the tendon strike and motor response. From this, they were able to estimate the sensorimotor conduction velocity. Additionally, a significant increase in the stretch reflex EMG amplitude response was observed when using the Jendrassik maneuver during the knee-jerk response. A student exit survey on the laboratory experience reported that the class found the module engaging and helpful for reinforcing physiological course concepts.ConclusionThis newly developed protocol not only allows BME students to explore physiological responses using natural and electrically-induced involuntary reflexes, but demonstrates that budget-friendly commercially available devices are capable of eliciting and measuring involuntary reflexes in an engaging manner. Despite some limitations caused by the equipment and students’ lack of signal processing experience, this new laboratory protocol provides a robust framework for integrating engineering and physiology in an applied approach for BME students to learn about involuntary reflexes, neurophysiology, and neural engineering.

【 授权许可】

CC BY