In vitro differentiation of W8B2+ human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity

Cellular & Molecular Biology Letters

Christophe Jayle¹  Nathalie Gaborit²  Zeina R. Al Sayed²  Oualid Ayad³  Aurélien Chatelier³  Christophe Magaud³  Jean-François Faivre³  Stéphane Sebille³  Charles-Albert Chapotte-Baldacci³  Patrick Bois³ 

[1] CHU of Poitiers chirurgie cardiaque et thoracique, , Poitiers Cedex 09, France;CNRS, INSERM, l’institut du thorax, Université de Nantes, 44000, Nantes, France;University of Poitiers Signalisation et Transports Ioniques Membranaires, EA7349, Poitiers Cedex 09, France;

关键词: W8B2 human cardiac stem cell;    Cardiac differentiation;    Calcium activity;    Oscillations;    Ion channels;    GCaMP;   

DOI  :  10.1186/s11658-020-00242-9

来源: Springer

【 摘 要 】

BackgroundHuman cardiac stem cells expressing the W8B2 marker (W8B2+ CSCs) were recently identified and proposed as a new model of multipotent CSCs capable of differentiating into smooth muscle cells, endothelial cells and immature myocytes. Nevertheless, no characterization of ion channel or calcium activity during the differentiation of these stem cells has been reported.MethodsThe objectives of this study were thus to analyze (using the TaqMan Low-Density Array technique) the gene profile of W8B2+ CSCs pertaining to the regulation of ion channels, transporters and other players involved in the calcium homeostasis of these cells. We also analyzed spontaneous calcium activity (via the GCaMP calcium probe) during the in vitro differentiation of W8B2+ CSCs into cardiac myocytes.ResultsOur results show an entirely different electrophysiological genomic profile between W8B2+ CSCs before and after differentiation. Some specific nodal genes, such as Tbx3, HCN, ICaT, L, KV, and NCX, are overexpressed after this differentiation. In addition, we reveal spontaneous calcium activity or a calcium clock whose kinetics change during the differentiation process. A pharmacological study carried out on differentiated W8B2+ CSCs showed that the NCX exchanger and IP3 stores play a fundamental role in the generation of these calcium oscillations.ConclusionsTaken together, the present results provide important information on ion channel expression and intrinsic calcium dynamics during the differentiation process of stem cells expressing the W8B2 marker.

【 授权许可】

CC BY